芪蓝抗毒饮治疗鸡传染性法氏囊病的剂量研究

比较了芪蓝抗毒饮三种剂量和中剂量三种给药次数对雏鸡实验性传染性法氏囊病的疗效。结果表明，中剂量和发病后该剂量每天给药1次、连续4天的疗效最好。     

引种草坪草的适应性评价及病害和草害

1997～1998年，对引自丹麦的草地早熟禾、黑麦草、高羊茅、羊茅、紫羊茅、匍匐剪股颖和白三叶等草坪草的品种在兰州市进行了适应性评价。观测、记载了草坪的成坪期、密度、盖度、颜色、质地、越冬率、越夏率、返青期、枯黄期、青缘期等性状；调查了病害的发病率，并对病株进行了病原物的分离、培养和鉴定，统计了各品种带菌率和真菌的分离率；调查了草坪建植与生长过程中的杂草种类和危害程度。结果表明：除Pichwick和Juventus黑麦草以外的所有参试品种均可在兰州地区种植。其中，白三叶的综合性状最好，具有质地柔滑、成坪较快、覆盖度高、枯黄期晚等特点；其次为ORNAMENTAL和SPORT2个混配型草种，成坪快，但抗病性较差；其余各品种建坪后表现好，但成坪速度较慢。草坪生长过程中的主要病害为交链孢叶斑病、离蠕孢叶斑病、德氏霉叶斑病、壳二孢叶枯病、壳二孢叶斑病、镰刀菌枯萎病和叶点霉叶斑病。苗期主要杂革为狗尾草和田旋花。     

马立克氏病毒单克隆抗体杂交瘤细胞株的建立与鉴定

以马立克氏病毒814株(MDV-814)作为免疫用抗原,建立了2个MDV特异性单克隆抗体(McAb)杂交瘤细胞株—4A6、3D7。鉴定结果表明;两株细胞所分泌的McAb均为IgM类免疫球蛋白,具有MDVⅠ型病毒特异性,无中和反应特性和沉淀反应特性。利用两种McAb对国内标准强毒MDV-京1株进行鉴定,肯定其为MDV-Ⅰ型毒株。     

D-Dimer Improves the Prognostic Value of Combined Clinical and Laboratory Data in Equine Gastrointestinal Colic

The discriminating ability of 15 parameters alone or in combinations, including results from analysis of plasma endotoxin, the Nycomed plasma D-Dimer test and phospholipase A₂, were analyzed to predict morbidity and mortality in equine gastrointestinal colic. Endotoxaemia was a characteristic feature of the colic horses. The problem of adequately predicting non-survivors among colic horses required several parameters to be included in the logistic model: if the “classical parameters”, (heart rate, respiratory rate, PCV, anion gap) were included in the model, addition of plasma D-dimer, phospholipase A₂, and Cl^- significantly improved the predictive value of the logistic model. Increasing heart rate and D-dimer together with decreasing chloride was a risk factor for nonsurvival. The sensitivity of this three-parameter logistic model to predict nonsurvival was 78% and specificity 77%. The Nycomed D-Dimer test is recommended as a horse-site test to predict disseminated intravascular coagulation and nonsurvival in equine colic.     

甜叶菊种传病害防治方法的研究

采用种子处理方法可显著提高种子发芽率、降低发病率；其中以药剂浸种效果最好；药剂处理以多菌灵＋福美双和多菌灵两个配方最佳。     

Bovine Leukocyte Adhesion Deficiency in Danish Holstein-Friesian Cattle: I. PCR Screening and Allele Frequency Estimation

Jergensen, C. B., J. S. Agerholm, J. Pedersen and P. D. Thomsen: Bovine leukocyte adhesion deficiency in Danish Holstein-Friesian Cattle I. PCR screening and allele frequency estimation. Acta vet. Scand. 1993,34,231-236.–A screening program for bovine leukocyte adhesion deficiency (BLAD) in Danish Holstein-Friesian cattle has been initiated. During the first months 1611 animals were tested by a PCR based assay. Of these animals 1256, 346, and 8 were assigned normal, BLAD carriers, and BLAD affected animals, respectively One bull, born as a co-twin, showed weak reaction for the BLAD allele on DNA isolated from leukocytes, but a normal genotype on DNA isolated from semen. Chromosome analysis showed that this bull was a blood chimaera. Estimation of the BLAD allele frequency upon the PCR test results showed that around 450 Danish calves born in 1991 might have been affected with the recessive disorder.     

USE OF ULTRASOUND-GUIDED FINE-NEEDLE ASPIRATION BIOPSY AND AUTOMATED CORE BIOPSY FOR THE DIAGNOSIS OF GASTROINTESTINAL DISEASES IN SMALL ANIMALS

The purpose of this study was to evaluate the usefulness of ultrasound-guided fine-needle aspiration biopsy and core biopsy in the diagnosis of infiltrative gastrointestinal diseases. Six dogs and seven cats with clinical signs of gastrointestinal disease underwent ultrasonography and intestinal lesions were identified. One or more ultrasound-guided fine-needle aspiration biopsy and/or core biopsy procedures were performed in each patient. Each patient also underwent one of the following additional procedures for comparison of results: 1) surgery (n=4), 2) endoscopy (n=2), 3) post mortem exam (n=3), and, 4) for lymphoma diagnosed with ultrasound-guided procedures, response to chemotherapy (n=4). Correct diagnoses were obtained in nine of the 13 patients, incorrect diagnoses in two of the 13 patients, and inconclusive diagnoses in two of the thirteen patients. Of the 16 total ultrasound-guided procedures performed, ten were confirmed as correct, three as incorrect, and three were non-diagnostic. Intestinal lesions with bowel wall thickness greater than 2.0 cm had a higher percentage of correct diagnoses than lesions of lesser wall thickness. Gastric lesions had a higher percentage of correct diagnoses than small and large intestinal lesions. Malignant lesions had a higher percentage of correct diagnoses than benign lesions. There were no complications. Ultrasound-guided gastrointestinal fine-needle aspiration biopsy and core biopsy appears to be a safe, accurate, and rapid procedure for use in the diagnosis of infiltrative gastrointestinal disease.     

复合酶高活性酵母对岭南黄肉鸡生产性能的影响

300只岭南黄肉鸡随机分为2组(每组设3个重复,每个重复50只),对照组饲喂基础日粮,试验组在基础日粮中用3%活性酵母替代3%豆粕。饲养试验结果表明:0～49日龄,平均末重、平均日增重、成活率试验组分别比对照组提高8.74%(P<0.05)、8.91%(P<0.05)和5.00%(P<0.05),试验组料蛋比比对照组下降11.25%(P<0.05)。代谢试验表明:能量表观利用率和粗蛋白质表观利用率试验组比对照组分别提高4.05%(P<0.05)和6.84%(P<0.05)。     

细菌耐药拮抗剂的研究

本文介绍了具有拮抗细菌耐药性作用的物质的研究进展情况，包括灭活酶抑制剂、药物渗透促进剂、外输泵抑制剂、细菌生物被膜抑制剂、抗菌药物增强剂、耐药质粒消除剂等。     

重迎茬亚麻生长发育障碍机制的研究

重迎茬亚麻减产是由多种因素综合作用的结果,亚麻重迎茬可使病害加重是最主要因素,重茬年限越久发病越严重;重迎茬造成了土壤营养元素的单一消耗,随着重迎茬年限的增加,土壤中速效氮、速效磷、速效钾等含量降低。